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geneticin  (Thermo Fisher)


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    Structured Review

    Thermo Fisher geneticin
    Geneticin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/geneticin/G418+disulfate%2C+Cell+Culture+Reagent/pm42249047-340-13-15
    Average 97 stars, based on 1 article reviews
    geneticin - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Endocytosis of the Saccharomyces cerevisiae Jen1 monocarboxylate-proton symporter under mildly alkaline conditions: a mechanism to prevent metabolite loss?
    Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS Strain construction All the S. cerevisiae strains generated in this work were constructed as follows: firstly, DNA fragments were amplified by PCR (Accuzyme DNA Polymerase, Bioline, or Supreme NZYProof DNA Polymerase, Nzytech) with specific oligonucleotides (listed in Table 2) using yeast genomic DNA or plasmid DNA. .. The resulting PCR products were introduced in S. cerevisiae cells, by following the polyethylene glycol (PEG)/lithium acetate (LiAc) protocol (36), and the resulting transformants were selected on geneticin (G418 disulfate, Alfa Aesar; J62671) or hygromycin B (Alfa Aesar; J60681) according to the resistance (R) marker present (geneticin for kanR and hygromycin for hghR). ..

    Article Title: Endocytosis of the Saccharomyces cerevisiae Jen1 monocarboxylate-proton symporter under mildly alkaline conditions: a mechanism to prevent metabolite loss?
    Article Snippet: All the S. cerevisiae strains generated in this work were constructed as follows: firstly, DNA fragments were amplified by PCR (Accuzyme DNA Polymerase, Bioline, or Supreme NZYProof DNA Polymerase, Nzytech) with specific oligonucleotides (listed in Table ) using yeast genomic DNA or plasmid DNA. .. The resulting PCR products were introduced in S. cerevisiae cells, by following the polyethylene glycol (PEG)/lithium acetate (LiAc) protocol [ ], and the resulting transformants were selected on geneticin (G418 disulfate, Alfa Aesar; J62671) or hygromycin B (Alfa Aesar; J60681) according to the resistance (R) marker present (geneticin for kan R and hygromycin for hgh R). ..

    Marker:

    Article Title: Endocytosis of the Saccharomyces cerevisiae Jen1 monocarboxylate-proton symporter under mildly alkaline conditions: a mechanism to prevent metabolite loss?
    Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS Strain construction All the S. cerevisiae strains generated in this work were constructed as follows: firstly, DNA fragments were amplified by PCR (Accuzyme DNA Polymerase, Bioline, or Supreme NZYProof DNA Polymerase, Nzytech) with specific oligonucleotides (listed in Table 2) using yeast genomic DNA or plasmid DNA. .. The resulting PCR products were introduced in S. cerevisiae cells, by following the polyethylene glycol (PEG)/lithium acetate (LiAc) protocol (36), and the resulting transformants were selected on geneticin (G418 disulfate, Alfa Aesar; J62671) or hygromycin B (Alfa Aesar; J60681) according to the resistance (R) marker present (geneticin for kanR and hygromycin for hghR). ..

    Article Title: Endocytosis of the Saccharomyces cerevisiae Jen1 monocarboxylate-proton symporter under mildly alkaline conditions: a mechanism to prevent metabolite loss?
    Article Snippet: All the S. cerevisiae strains generated in this work were constructed as follows: firstly, DNA fragments were amplified by PCR (Accuzyme DNA Polymerase, Bioline, or Supreme NZYProof DNA Polymerase, Nzytech) with specific oligonucleotides (listed in Table ) using yeast genomic DNA or plasmid DNA. .. The resulting PCR products were introduced in S. cerevisiae cells, by following the polyethylene glycol (PEG)/lithium acetate (LiAc) protocol [ ], and the resulting transformants were selected on geneticin (G418 disulfate, Alfa Aesar; J62671) or hygromycin B (Alfa Aesar; J60681) according to the resistance (R) marker present (geneticin for kan R and hygromycin for hgh R). ..

    Stable Transfection:

    Article Title: Synthesis, in silico and in vitro evaluation of N-phenylbenzimidazole derivatives targeting muscarinic acetylcholine receptors.
    Article Snippet: .. Chinese hamster ovary (CHO-K1) cells stably expressing the hM1hM5 receptors were obtained from Missouri University of Science and Technology cDNA Resource Center (Cell Catalog#: CEM1000000, CEM2000000, CEM3000000, CEM4000000, CEM5000000) and cultivated in Gibco Ham's F-12 Nutrient Mixture supplemented with 10% (v/ v) GibcoTM FBS, 250 mg/mL Geneticin® (G418, Thermo Fisher, Waltham, MA), and L-glutamine (1%; 200 nM) at 37 ◦C in a 5% CO2 humidified atmosphere. ..

    Expressing:

    Article Title: Synthesis, in silico and in vitro evaluation of N-phenylbenzimidazole derivatives targeting muscarinic acetylcholine receptors.
    Article Snippet: .. Chinese hamster ovary (CHO-K1) cells stably expressing the hM1hM5 receptors were obtained from Missouri University of Science and Technology cDNA Resource Center (Cell Catalog#: CEM1000000, CEM2000000, CEM3000000, CEM4000000, CEM5000000) and cultivated in Gibco Ham's F-12 Nutrient Mixture supplemented with 10% (v/ v) GibcoTM FBS, 250 mg/mL Geneticin® (G418, Thermo Fisher, Waltham, MA), and L-glutamine (1%; 200 nM) at 37 ◦C in a 5% CO2 humidified atmosphere. ..

    other:

    Article Title: MORC3 represses a tandem repeat enhancer to regulate interferon.
    Article Snippet: The two homology donor plasmids, one with hygromycin B and the other with neomycin cassettes, and the sgRNA plasmid were electroporated into BLaER1 cells using a BioRad GenePulser device (265 V; 975 μF; 700 ohm).

    Article Title: Intracellularly delivered nanobody targeting the nucleocapsid protein effectively inhibits porcine deltacoronavirus replication.
    Article Snippet: In-Fusion Snap Assembly Master Mix and PrimeSTA Max DNA Polymerase were obtained from Takara Biomedical Technology (Beijing) Co., Ltd. Trizol reagent was purchased from Thermo Fisher Scientific Inc. Puromycin, Geneticin (G418), doxycycline, and polybrene were purchased from GlpBio Technology (Shanghai) Co., Ltd. All-In-One 5×RT Master Mix was supplied by Applied Biological Materials In (abm).

    Cell Culture:

    Article Title: Self-healing chitosan-catechol hydrogel with tailored self-assembled fibrillar or micellar structures for biomedical applications
    Article Snippet: .. Cells were cultured in a 1:1 mixture of highglucose Dulbecco's modified Eagle's medium (HG-DMEM; 4.5 g/L glucose, Gibco, USA), 400μg/mL geneticin (G418, Invitrogen, USA), and Ham's F-12, supplemented with 10% fetal bovine serum (FBS; Gibco, USA) and 1% penicillin–streptomycin–amphotericin (PSA; Gibco, USA). ..

    Article Title: Machine learning classification of normal and malignant cells on the basis of their viscoelastic properties.
    Article Snippet: .. This cell line was cultured in RPMI medium with L-glutamine, HEPES buffer and phenol red (GibcoTM, Thermo Fisher Scientific Inc.), as well as 10% fetal bovine serum (FBS, GibcoTM, Thermo Fisher Scientific Inc.), 1% penicillin-streptomycin (GibcoTM, Thermo Fisher Scientific Inc.) and 1% geneticin (G418, GibcoTM, Thermo Fisher Scientific Inc.). ..

    Modification:

    Article Title: Self-healing chitosan-catechol hydrogel with tailored self-assembled fibrillar or micellar structures for biomedical applications
    Article Snippet: .. Cells were cultured in a 1:1 mixture of highglucose Dulbecco's modified Eagle's medium (HG-DMEM; 4.5 g/L glucose, Gibco, USA), 400μg/mL geneticin (G418, Invitrogen, USA), and Ham's F-12, supplemented with 10% fetal bovine serum (FBS; Gibco, USA) and 1% penicillin–streptomycin–amphotericin (PSA; Gibco, USA). ..



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